Journal: Communications Biology

Article Title: tRF16 affects NFKBIA stability and promotes osteoarthritis progression by regulating ALKBH5 expression in m6A-dependent manner

doi: 10.1038/s42003-025-08299-y

Figure Lengend Snippet: A , B Volcano and heat maps of smallRNAs differentially expressed in cartilage tissues of three normal subjects and three OA patients analyzed using Arraystar Human SmallRNA Expression Microarray; C flow diagram of a rat OA model established by meniscectomy. The illustration is created by Biorender.com; D microCT confirms arthritic symptoms in rats at 16 weeks postoperatively; E the Tb.N, Tb.sp and BV/TV value according to the micro-CT; F – H , HE, PAS, and SAFG staining to detect pathological structural alterations in the cartilage tissues of rat knee joints; I RT-qPCR to detect tRF16 expression in the cartilage tissues of rats at 0, 4, 8, 12, and 16 weeks postoperatively; J RT-qPCR to detect the mRNA levels of Aggrecan, COL2A1, MMP1, and MMP13 in the cartilage tissues of rats; K the correlation between tRF16 expression in the knee joints of OA rats at week 16 and the Mankin score. Each group contained 6-8 rats; the data were presented as dot and whsikers and statistically analyzed using Student t -test or 2-way ANOVA, followed by Tukey’s multiple comparison test for post hoc tests, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: In the present study, we used Arraystar Human SmallRNA Expression Microarray to analyze the differentially expressed small RNA in cartilage tissue and observed significantly overexpressed tRF16 in OA patients.

Techniques: Expressing, Microarray, Micro-CT, Staining, Quantitative RT-PCR, Comparison

Journal: Toxicological Sciences

Article Title: Chronic Hexavalent Chromium Exposure Upregulates the RNA Methyltransferase METTL3 Expression to Promote Cell Transformation, Cancer Stem Cell-Like Property, and Tumorigenesis

doi: 10.1093/toxsci/kfac023

Figure Lengend Snippet: Total RNA N6-methyladenosine (m6A) modification levels are significantly increased in chronic hexavalent chromium [Cr(VI)] exposure-transformed human bronchial epithelial cells and chronic chromate-exposed mouse lung tissues. A, The heatmap from m6A microarray analysis showing the extent of total messenger RNA m6A methylation in passage-matched control cells (BEAS-2B-Control) and chronic Cr(VI) exposure-transformed cells [BEAS-2B-Cr(VI)]. B and C, Relative total RNA m6A levels measured by using the EpiQuik m6A RNA Methylation Quantification Kit. The total RNA m6A levels in Cr(VI)-transformed cells (B) or chromate-exposed mouse lung tissues (C) are expressed relative to the passage-matched control cells (means ± SD, n = 3) (B) or vehicle control-exposed mouse lungs (means ± SD, n = 6) (C), respectively. *p < .05.

Article Snippet: Human m 6 A RNA modification microarray analysis Total RNAs from chronic Cr(VI) exposure-transformed BEAS-2B cells [BEAS-2B-Cr(VI)] and the passage-matched control BEAS-2B cells (BEAS-2B-Control) were submitted to Arraystar Inc. (Rockville, Maryland) for human m 6 A RNA modification microarray analysis.

Techniques: Modification, Transformation Assay, Microarray, Methylation, Control

Journal: bioRxiv

Article Title: Cannabinoids shift the basal ganglia miRNA m 6 A methylation profile towards an anti-inflammatory phenotype in SIV-infected Rhesus macaques

doi: 10.1101/2024.10.11.614514

Figure Lengend Snippet: A) Percent mature and star miRNAs with m 6 A methylation marks. B-D) m 6 A methylation patterns in each treatment group. E-G) Heatmap of m6A methylation profile versus miRNA abundance.

Article Snippet: We have completed the Arraystar Human N6-methyladenosine (m6A) small RNA modification microarray analysis of the samples you submitted.

Techniques: Methylation

Journal: bioRxiv

Article Title: Cannabinoids shift the basal ganglia miRNA m 6 A methylation profile towards an anti-inflammatory phenotype in SIV-infected Rhesus macaques

doi: 10.1101/2024.10.11.614514

Figure Lengend Snippet: A) Neuroinflammation network molecules associated with the with miRNAs with m6A hypomethylation (left) or hypermethylation (right).

Article Snippet: We have completed the Arraystar Human N6-methyladenosine (m6A) small RNA modification microarray analysis of the samples you submitted.

Techniques: